Template Switching Oligo

Template Switching Oligo - To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. However, if a large fraction of the library contains the. The tso adds a common 5'. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. Template switching oligo and capturing oligo (dt) primer are designed for use in template switching reverse transcription reactions, while preamplification primer is used in subsequent. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during reverse. The mix contains a unique rt. We recommend a tso with rgrgrg at the 3′ end.

In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. The mix contains a unique rt. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. The template switching rt enzyme mix and accompanying reaction buffer enable efficient template switching activity in a reverse transcription reaction. We recommend a tso with rgrgrg at the 3′ end. However, if a large fraction of the library contains the. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of. The tso adds a common 5'. The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during reverse.

Template Switch Oligo

A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of. The template switching rt enzyme mix and accompanying reaction buffer enable efficient template switching activity.

(A) The TS mechanism is used for first strand cDNA synthesis. First, an

A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of. The mix contains a unique rt. In general, different tsos can be used including tsos.

Fig. S12 Schematic representation of the tested oligodT / TSO

However, if a large fraction of the library contains the. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. The mix contains a unique rt. We recommend a tso with rgrgrg at the 3′ end. Template switching oligo and capturing oligo (dt) primer are designed for use.

What Is A Template Switching Oligonucleotide

The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during reverse. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. Template switching oligo and capturing oligo (dt) primer are designed for use in template.

AccelerRT® 5G Template Switching RT Enzyme Mix GeneCopoeia™

To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of. Template switching oligo and capturing oligo (dt) primer are designed for use in template switching reverse transcription reactions, while preamplification primer is used in subsequent. The tso adds a common 5'. However, if a large fraction of the.

(A) First strand cDNA is initiated by priming with an oligo dT primer

Template switching oligo and capturing oligo (dt) primer are designed for use in template switching reverse transcription reactions, while preamplification primer is used in subsequent. To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of. However, if a large fraction of the library contains the. We recommend a.

Model of cDNA synthesis that occurs in the absence (A) and presence (B

Template switching oligo and capturing oligo (dt) primer are designed for use in template switching reverse transcription reactions, while preamplification primer is used in subsequent. We recommend a tso with rgrgrg at the 3′ end. The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during reverse. The template.

Template switching oligos (TS oligos, TSOs) for cDNA library

A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. The mix contains a unique rt. However, if a large fraction of the library contains the. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse.

Tuning 5’ to internal read proportions and template switching oligo PCR

To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of. The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during reverse. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added.

Template Switching Oligo

The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. The mix contains a unique rt. However, if a large fraction of the library contains the. The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during.

The Template Switching Rt Enzyme Mix And Accompanying Reaction Buffer Enable Efficient Template Switching Activity In A Reverse Transcription Reaction.

We recommend a tso with rgrgrg at the 3′ end. Template switching oligo and capturing oligo (dt) primer are designed for use in template switching reverse transcription reactions, while preamplification primer is used in subsequent. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of.

The Tso Adds A Common 5'.

The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during reverse. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. However, if a large fraction of the library contains the. The mix contains a unique rt.